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Thursday, August 4, 2011

Comparison of Matrix Effects in HPLC-MS/MS

Jet C. Van De Steene and Willy E. Lambert
Laboratory of Toxicology, Ghent University, Ghent, Belgium
When developing an LC-MS/MS-method matrix effects are a major issue. The effect of
co-eluting compounds arising from the matrix can result in signal enhancement or suppression.
During method development much attention should be paid to diminishing matrix
effects as much as possible. The present work evaluates matrix effects from aqueous
environmental samples in the simultaneous analysis of a group of 9 specific pharmaceuticals
with HPLC-ESI/MS/MS and UPLC-ESI/MS/MS: flubendazole, propiconazole, pipamperone,
cinnarizine, ketoconazole, miconazole, rabeprazole, itraconazole and domperidone. When
HPLC-MS/MS is used, matrix effects are substantial and can not be compensated for with
analogue internal standards. For different surface water samples different matrix effects are
found. For accurate quantification the standard addition approach is necessary. Due to the
better resolution and more narrow peaks in UPLC, analytes will co-elute less with interferences
during ionisation, so matrix effects could be lower, or even eliminated. If matrix effects
are eliminated with this technique, the standard addition method for quantification can be
omitted and the overall method will be simplified. Results show that matrix effects are almost
eliminated if internal standards (structural analogues) are used. Instead of the time-consuming
and labour-intensive standard addition method, with UPLC the internal standardization can
be used for quantification and the overall method is substantially simplified. (J Am Soc Mass
Spectrom 2008, 19, 713–718) © 2008 American Society for Mass Spectrometry

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